摘要
选择两段血小板反应素(TSP-1)中抑制血管再生的活性片段,设计两对引物,使用RT-PCR的方法从人血细胞中进行克隆并对获得片段进行测序验证.克隆片段大小分别为723和522bp,命名为TSP-1-1和TSP-1-2.利用原核表达载体pET-29a获得大肠杆菌重组子,重组子经过IPTG诱导以包涵体的形式表达相应的多肽片段.再对包涵体进行体外溶解、纯化,得到了目的多肽.
Two pairs of primers were designed according to the sequence of human thrombospondin-1 （TSP-1）. Two active fragments of TSP-1, called TSP-1-1 and TSP-1-2, were obtained by using reverse transcription-polymerase chainreaction （RT-PCR） from blood samples. The sizes of those two fragments were found to be 723bp and 522bp respectively. They were inserted into pET-29a vector to get E. coli recombinants. Target peptides were found in inclusion body after IPTG inducement. Dissolving inclusion body in PBS were got by soluble TSP-1-2 peptides.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第5期1047-1050,共4页
Journal of Sichuan University(Natural Science Edition)
