摘要
目的观察黄芪注射液对N甲基N亚硝脲(MNU)诱导SD大鼠视网膜光感受器细胞凋亡的保护作用。方法将出生后47d的♀SD大鼠随机分为正常对照组、模型组及黄芪3个剂量组,腹腔注射给予生理盐水或黄芪,每日1次,并于给药第4日腹腔注射MNU60mg·kg-1,然后分别于不同时间摘取动物眼球。形态学分析测量视网膜的总厚度;TUNEL法和转录因子分析检测光感受器细胞凋亡和胞核内NFkBp65活性。结果黄芪注射液可显著增加周边视网膜总厚度、降低MNU引起的光感受器细胞凋亡指数,并增加视网膜组织细胞胞核内NFkBp65的活性。结论黄芪注射液对MNU引起的视网膜损伤有一定的保护作用。
AIM ：To observe the protective effect of. Astragalus injection on N-methyl-N-nitrosourea （ MNU ）- induced photoreceptor apoptosis in SD rats. METHODS ： Female 47 - day SD rats were randomly divided into control group, model group and three dose groups of Astragalus. Saline or Astragalus was injected intraperitoneally into rats once a day. On 4^th day after treatment, the rats were injected intraperitoneally by 60 mg· kg^-1 MNU and the eyes were taken in different phases. Retinal damage was evaluated based on retinal thickness. The apoptotic index of the photoreceptor cells and the activity of nuclear NF-kB p65 were detected by TUNEL labeling and Transcription Factor Assay Kit, respectively. RESULTS ： Astragalus injection could remarkly increase total thickness of peripheral retina and decrease apoptotic index of photoreceptor cells induced by MNU and increase the activity of nuclear NF-kB p65. CONCLUSION：Astragalus injection partially protects against MNU-induced retinal damage.
出处
《中成药》
CAS
CSCD
北大核心
2005年第9期1065-1067,共3页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(30300467)
河南省科技攻关项目(0523030700)
