摘要
选择杂种犬７只，观察一氧化氮合成前体──Ｌ－精氨酸对犬急性缺氧性肺动脉高压的影响，并在整体动物及培养的人脐带静脉内皮细胞中观察Ｌ－精氨酸对内皮素－１（ＥＴ－１）分泌的作用。结果表明Ｌ－精氨酸可降低麻醉犬急性缺氧时的肺动脉压及股动脉压，其作用持续约３０ｍｉｎ，但对犬血浆ＥＴ－１水平却无影响；在培养的人脐带静脉内皮细胞中，不同浓度的Ｌ－精氨酸对培养４或２４ｈ的内皮细胞分泌ＥＴ－１水平也无影响。提示Ｌ－精氨酸可拮抗急性缺氧性肺动脉高压但不影响ＥＴ－１的分泌。
This study is aimed to ivestigate the effects of L-arginine,a precursor of the formation of nitric oxide,on acute hypoxic pulmonary hypertension in vivo and on production of endothelin-1 both in vivo and in cultured endothelial cells.In mechanically ventilated anesthetized dogs(n=7),L-arginine(0.5g/kg)reduced the mean pulmonary arterial pressure and femoral arterial pressure during hypoxic ventilation and its action lasted for about 30 minutes.Meanwhile,plasma endothelin-1 in the pulmonary and femoral artery had no remarkable change.In cultured endothelial cells from umbilical veins,different concentrations of L-arginine had no influence on endothelin-1 level of culture medium in 4 or 24 hours after the addition of L-arginine.These results indicate that L-arginie can decrease the pulmonary arterial pressure during acute hypoxia,which may be associated with the increase of nitric oxide production.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1996年第3期214-218,共5页
Acta Academiae Medicinae Sinicae
基金
国家"八五"攻关项目基金
卫生部青年基金
关键词
精氨酸
缺氧
肺动脉高压
内皮素
内皮细胞
L-arginine
hypoxia
pulmonary hypertension
endothelin-1
endothelial cells
