摘要
应用ＰＣＲ技术扩增并克隆恶性疟原虫海南、云南、安徽３个地理株ＭＳＡ１第二区基因，测定并分析其基因序列。结果表明，海南、云南、安徽株扩增片段长度为４２３ｂｐ，基因序列完全相同，我国虫株ＭＳＡ１与ＷＥＬＬＣＯＭＥ株最为相似，存在ＭＡＤ和Ｋ１两种等位基因型的基因内重组现象。
Gene encoding the second region of merozoite surface antigen(MSA1-R2)of three Chinese isolates of Plasmodium falciparum from Hainan,Yunnan and Anhui were amplified by PCR,cloned into M13 and sequenced.Comparison among MSA1-R2 sequences from different isolates of P.falciparum shows that there is no difference among the three Chinese isolates.The sequence of MSA1-R2 of the Chinese isolates are highly homologous with that of WELLCOME from West Africa.The sequence flanking the upstream of the tandem repeats is exactly the same as that of MAD-20,but sequence downstream the repeats exhibit the characteristics of K1 allele,indicating that intragenic recombination of the two MSA1 allelic families exists in the Chinese isolates.
出处
《中国医学科学院学报》
CSCD
北大核心
1996年第3期161-165,共5页
Acta Academiae Medicinae Sinicae
