摘要
以湿地松组培嫩梢为外植体诱导不定根。研究结果表明,基本培养基及其中附加生长素的种类及浓度对不定根的分化有较大影响,改良1/2GD是湿地松嫩梢生根的较优基本培养基,其次为1/2DCR,1/2MS;嫩梢接种在改良1/2GD+IBA2.0的培养基中培养30d后,其不定根诱导率最高达51.6%。在改良1/2GD+NAA0.05的培养基中不定根诱导率最高达59.3%,NAA与IBA组合较单独使用具有更佳的生根效果,嫩梢接种1/2GD+NAA0.05+IBA1.0上30d后最高生根率达81.3%,但NAA与6-BA或活性炭(AC)组合均不利于湿地松嫩梢的生根。生根小植株转移至无激素的改良1/2GD培养基中,不定根伸长较快。在适宜的温湿度条件下,再生植株移栽成活率达85%。
Roots induction was made with the explants of the shoots for Pinus elliottii obtained by tissue culture. Results showed that the basal medium and the categories and concentrations of additive plant growth regulators all had great effect on the differential of adventitious roots. Modified 1/2GD medium was proved superior to 1/2DCR and 1/2MS. Roots formed when excised shoots were cultivated on Modified 1/2GD medium supplemented with 2.0 mg·L^-1 IBA or 0.05 mg · L^-1 NAA for 30 days, and the highest inducing rates of roots were 51.6% and 59.3% , respectively. While the combination of NAA （0.05 mg · L^-1） and IBA （1.0 mg·L^-1） had better effect on rooting, and the rooting rate could reach 81.3% after excised shoots were cultivated on 1/2GD ＋ NAA0.05 ＋ IBA1.0 medium for 30 days. The medium containing NAA with 6 - BA or AC （Activated Charcoal） did not support root formation. All the plantlets were transferred to hormone -free modified 1/2GD medium for roots elongation. The survival rate was up to 85% before the regenerated plantlets were moved to the greenhouse.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2005年第5期15-18,共4页
Journal of Northeast Forestry University
关键词
湿地松
组织培养
不定根诱导
植株再生
Pinus elliottii
Tissue culture
Roots induction
Plantlet regeneration
