摘要
应用聚丙烯酰胺凝胶等电聚焦电泳技术，对雄激素受体亚群进行了研究。结果发现，前列腺增生组织中有三种雄激素受体亚型，等电点（ＰＩ）分别在６．５、６．０和５．３。其中９５％（１９／２０）的标本含有Ｐ１６．５、５．３亚型，４５％（９／２０）的标本含有ＰＩ６．５、６．０和６．３亚型，５０％（１０／２０）的标本含有ＰＩ６．０亚型，５％（１／２０）的标本只有Ｐ１５．３亚型。３例前列腺癌标本中，２例有Ｐ１６．５、６．０、５．３三种亚型，１例有６．５、６．５两种亚型。氘标的双氢睾酮（ＤＨＴ）与三种受体亚型的结合，可以被非氚标的ＤＨＴ，睾酮（Ｔ）竞争抑制，而孕酮、雌二醇和己烯雌酚对此却无竞争抑制作用。
With isoelectric focusing technique,the heterogenous nature of androgen receptors(AR)in benign hyperplastic and inalignant prostatic tissues was studied,AR isoforms could be detected at PI values of 6.5,6.0 and 5.3.In 19 of the 20 samples,the isoform was detected at PI 6.5 and 5.3;9 of the 20 expressed all the isoforms;10 of the 20 expressed the PI 6.0 isoform and 1 expressed the PI 5.3 isoform. 2/3 of the malignant tissues contained all the 3 isoforms and 1/3 contained the PI 6.5,6.0 isoforms. Binding of(3H)dihydrotestosterone to the 3 isoforms could be inhibited by 100 fold excess of dihydrotestosterone and testosterone but not by progesterone,oestradiol of diethylstibaestrol.Genesis of these isoforms has not yet been clear but they may play an important role in tissue sensitivity to the androgens.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1996年第6期368-370,共3页
Chinese Journal of Urology
关键词
前列腺增生症
前列腺肿瘤
雄激素受体
Benign prostatic hyperplasiam,Prostatic neoplasms,Androgen receptor
