摘要
Genetic relationship of 12 species of Section Strobus was analyzed with ISSR markers. 117 loci were detected with 12 ISSR primers. Percentage of polymorphic bands (PPB) varied from 5.93% to 19.92%. P. pumila had the highest levels of genetic differentiation and P. flexilis had lowest. Total genetic diversity (Hr) of 12 species in Section Strobus was 26.21%, of which intraspecific genetic diversity (Hs) was 7.66%, and interspecific genetic diversity (DST) was 18.55%, and the genetic variation in interspecies accounted for 70.78% of the total genetic diversity. According to the cluster results of genetic distance, the 12 species were classified into two groups. The first group included P. griffithii, P.armandi, P. fenzeliana, P. kwangtungensis, P. strobus, P. monticola and P. wangii. The second group included P. albicaulis, P pumila, P. flexilis, P. sibirica and P koraiensis.
节 Strobus 的 12 种类的基因关系与 ISSRmarkers 被分析。117 loci 与 12 份 ISSR 教材被检测。多态的乐队(PPB ) 的百分比从 5.93% ~ 19.92% 变化了。P。pumila 有基因区别和 P 的高水平。flexilishad 最低。在节 Strobus 的 12 种类的全部的基因差异(H_T ) 是 26.21% ,基因差异(H_S ) 是的 whichintraspecific 7.66% ,并且种间的基因差异(D_( 圣))was18.55% ,和遗传变异在内部种类说明了 70.78% 全部的基因差异。根据遗传距离的簇结果, 12 种类被分类进二个组。第一个组包括了 P。griffithii, P。armandi, P。fenzeliana, P。kwangtungensis, P。strobus, P。monticola 和 P。wangii。第二个组包括了 P。albicaulis, P。pumila, P.flexilis, P。sibirica 和 P。koraiensis。
基金
The study was supported by Introducing Overseas Ad-vanced Agriculture Science and Technology Program of China (948 Program) (2001-31)