摘要
目的建立哮喘宁胶囊中盐酸麻黄碱的含量测定方法。方法采用高效液相色谱法,Hypersil ODS填料色谱柱(4.6×250 mm,5μm);柱温40℃;进样量10μL;流动相:乙腈-0.05 mol/L磷酸二氢钠溶液(磷酸调节pH值至2.7)(22︰78);流速:1.0 mL/min;检测波长:205 nm。结果盐酸麻黄碱在0.1005￣0.5025μg范围内,进样量与峰面积积分值之间线性关系良好,r=0.9993;平均回收率为103.1%,RSD=1.81%。结论该方法简便准确,重现性好,可用于哮喘宁胶囊的质量控制。
Objective To develop a HPLC method for the determination of ephedrine hydrochloride in Xiaochuanning capsules. Methods A Hypersil ODS column was used. The mobile phase was acetonitrile-water solution of 0. 05 mol/L NaH2PO4 （pH2. 7, 22：78）. The flow rate was 1.0 mL/min and column temperature was 40 ℃. Detcted wavelength was 205 nm. Results A good linearity was in the range of 0. 1005 - 0. 5025 μg （r = 0. 9993）. The average recovery was 103. 1%, RSD=1. 81%. Conclusion The method is simple, accurate, and can be used for the quality control of Xiaochuanning capsules.
出处
《中药新药与临床药理》
CAS
CSCD
2005年第5期359-361,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
