摘要
海甘蓝种子在附加有０～１０ｍｇ／Ｌ６—ＢＡ＋０．１ｍｇ／ＬＮＡＡ的ＭＳ培养基中，当６—ＢＡ为２～５ｍｇ／Ｌ时，幼苗生长健壮．采用ＭＳ＋２～４ｍｇ／Ｌ６—ＢＡ＋０．１ｍｇ／ＬＮＡＡ培养基可从无菌苗茎尖诱导大量丛生芽，２～３周后，平均每个茎尖可诱导５．１～５．５个丛生芽，６—ＢＡ大于４ｍｇ／Ｌ，会导致丛芽黄化．继代培养时，４ｍｇ／Ｌ６ＢＡ＋０．１ｍｇ／ＬＮＡＡ配合使用效果较佳，繁殖系数高达１０．４．１／２ＭＳ＋０．５ｍｇ／ＬＩＢＡ丛芽的生根效果较好，保湿组培苗的成活率可达９５％，再生植株在自然条件下能正常开花结实．
Abstract Crambe aby ssinica seeds were cultured on MS basal medium with 0～10mg/L6 Benzylaminopurine (BA) + 0. 1mg/L naphthaleneacetic acid (NAA), and robust seedlings were obtained on MS with 2～5mg/L 6BA. When the sterile seedlings were transplanted to medium MS+2～4mg/L 6BA +0. 1mg/L NAA,a lot of dark green multiple shoots were induced. After 2～3 weeks'culture, the means5.1～5. 5 shoots were produced from each stem apex, whereas more than 4mg/L 6BA caused the shoots to yellow. The shoots were subcultured and quickly propagated on MS + 4mg/L 6BA + 0. 1mg/L NAA. The propagation coefficient reached 1 0. 4. After transplanted to medium 1 /2MS + 0. 5mg/L IBA .the shoots began to root in 15 days. Survival rate of cultured plantlets reached 95% under enough humidity.The regenerated plantlets could normally flower and fruit in the field.
出处
《中国油料》
CAS
CSCD
北大核心
1996年第2期4-6,共3页
基金
四川省应用基础资助项目
