摘要
用保温法和裂解法可以有效提取Ｆ_(１９８７)新菌毛抗原蛋白质；该菌毛蛋白经区带电泳显示主、次两个组分，在０．０６ｍｏｌ／ＬｐＨ８．６巴比妥缓冲液电泳条件下，均向阳极泳动；免疫电泳显示两条沉淀线；其分子量为１９１００Ｄａ；等电点为３．０；由天门冬氨酸、苏氨酸、丝氨酸、谷氨酸、甘氨酸、丙氨酸、缬氨酸、异亮氨酸、亮氨酸、酪氨酸、苯丙氨酸、赖氨酸、组氨酸、精氨酸、脯氨酸、胱氨酸等１６种氨基酸组成。
F_(1967) new fimrial antigen Protein might be effectively drawed by the heat preserva-tion method and splitting method.The fimbrial protein by electrophoresis zone showedtwo parts,moving towards the positive pole in 0.06mol/L pH8.6 veronal buffer elect-rophoresis;Immunoelectrophoresis showed two precipitate lines;Its molecular weightwas 19100Da;Its isoelectric point was 3.36;It was made up of 16 amino acids,theyare aspartic acid,threonine,serine,glutamic acid,glycine,alarine,valine,isoleucine,leucine,tyrosine,phenylalanine,lysine,histidine,arginine,proline,and cystine.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1996年第4期377-380,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金
