摘要
用对一硝基苯基磷酸二钠法、聚丙烯酰胺凝胶圆盘电泳法，对三种母奶水牛—摩拉（Ｍｕｒｒａ）、尼里（Ｎｉｌｉ－Ｒａｖｉ）及三品系杂交［Ｎｉ♂×（Ｍｕ♂×Ｎａｎｎｉｎｇ♀）♀］水牛血清ＡＬＰ总活力、同工酶及其与产奶量的关系进行了测定和分析研究。结果表明：ＡＬＰ总活力为，三品杂（０．０６３６μｍｏｌ／ｍｉｎ·ｍｌ）＞摩拉（０．０６３μｍｏｌ／ｍｉｎ·ｍｌ）＞尼里（０．０４３０μｍｏｌ／ｍｉｎ·ｍｌ）；各品种内高、低产奶量比较为，高产＞低产。其同工酶的特点是，共有三种同工酶，其中尼里为ＡＬＰ＿２》ＡＬＰ＿１＞ＡＬＰ＿３、三品杂和摩拉为ＡＬＰ２》ＡＬＰ＿３＞ＡＬＰ＿１，但在尼里也出现了ＡＬＰ＿３＞ＡＬＰ＿１、在三品杂和摩拉也出现了ＡＬＰ＿１＞ＡＬＰ＿３的情况；在各品种内高、低产奶量比较是，ＡＬＰ＿２为高产＞低产、ＡＬＰ＿１和ＡＬＰ＿３为低产＞高产。并直接证实了两性载体电解质为水牛血清ＡＬＰ的强烈抑制剂。
The total activity of serum alkaline phosphatase(ALP)and its isoenzymes,therelation of the total activity and ALP isoenzymes to the milk output in the buffalocows of Murra,Nili Ravi and hybrid [Ni♂×(Mu♂×Nanning♀)♀]were studiedwith the methods of pnitrophenylphosphatic disodium salt and polyaccrylalnide disc gelelectrophoresis.Results showed that the total activity of serum ALP were 0.0636μmol/min.ml in hybrid animals,0.0634μmol/min.ml in Murra and 0.0430μmol/min.mlinNili-Ravi.Within a breed ALP was higher in the animals with higher milk output thanthose with lower milk output.Three isoenzymes of ALP were proved in the study.Theircommon situation in serum were ALP2>>ALP1>ALP3 in Nili-Ravi and ALP2 >>ALP3>ALP1 in Murra and hybrid animals.In a few cases there were ALP3>ALP1 in Nili-Raviand ALP1>ALP3 in Murra and hybrid animals.Within a breed ALP2 was higher in ani-mals with greater milk output than those with lower milk output and this was reversedin ALP1 and ALP3.This study reveals that ampholyte carrier is a strong inhibitor toserum ALP in huffalo cow.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1996年第4期303-307,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
水牛
血清
碱性磷酸酶
Buffalo cows,Serum,ALP,Isoenzyme,Inhibitor
