摘要
以同源四倍体高粱品系四甜－１、保持系四６２２Ｂ、约翰逊草以及同源四倍体不育系四６２２Ａ×约翰逊草和四甜－１×约翰逊草的杂种后代为材料，分别在１／２ＭＳ、Ｎ６和Ｗ１４培养基上进行花药培养。四６２２Ａ×约翰逊草和四甜－１×约翰逊草的杂种后代在Ｗ１４培养基上产生了愈伤组织，同源四倍体亲本６２２Ｂ在Ｎ６培养基上产生了愈伤组织。对一定的培养基，愈伤组织的诱导主要取决于基因型。另外，对Ｗ１４培养基设置了加活性碳与不加活性碳两组试验，结果表明，活性碳可有效控制花药中产生的有害色素的影响。
Two Lines,including autotetraploid 622B and autotetraploid Tian-1;Johnsongrass (S. halepens) and two hybrids, one autoteraploid 622A×Johnsongrass and one autotetraploid Tian-1×Johnsongrass were tested for their response to anther culture.Among the three media tested (1/2MS, W14, N6), W14 medium was effective in inducing anther calli from the two hybrids while N6 medium was effective in inducing anther calli from autotetraploid 622B. For a given medium, callus induction was obviously genotype dependent. Besides,W14 medium was divided into two groups:one with active carbon and the other without.The result showed that the active carbon could control the influence of the harmful pigment.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
1996年第3期56-59,共4页
Journal of Hebei Agricultural University
基金
国家自然科学基金
关键词
高梁
约翰逊草
远缘杂交
同源四倍体
花药培养
Sorghum
Johnsongrass(S.halepens)
Wide hybridization
Autotetraploid
Anther culture
