摘要
以芦丁为标样，用分光光度法分析了２０个不同桑品种春秋叶的黄酮类化合物总量，不同品种桑叶中，春叶黄酮含量为１１．７１～２６．６１ｍｇ／ｇ，秋叶为９．８４～２９．６０ｍｇ／ｇ．鲜叶直接测定，风干和烘干３种预处理比较，鲜叶黄酮含量最高．热水、３０％乙醇水溶液经半小时回流，提取率大于９５％．用高效液相色谱法测定了桑叶黄酮类化合物的组成，结果表明，桑叶中至少含有４种黄酮化合物。
Flavonoid content in mulberry leaves from 20 species is deteminded by spectrophotometic method using rutin as standard sample.Flavonoid content in spring leaves varies from 11.71 to 26.61 mg/g according to species and from 9.84 to 29.60 mg/g in autumn leaves.The flavonoid extract rate with three different sample pretreatments is stuied.The fresh leaves have the highest flavonoid content.Flavonoid extract rate is over 95% using hot water and 30% alcohol as solvent respectively.Flavonoid ingredients are distinguished and detemined with HPLC.The results show that mulberry leaves contain four flavonoids,two of which are rutin and quercetin.
基金
浙江省教委科研基金
