摘要
目的观察组胺H2受体拮抗剂雷尼替丁对哮喘小鼠T辅助细胞(Th1/Th2)功能性平衡的影响。方法40只BALB/C小鼠分为对照组、哮喘组、雷尼替丁2mg/kg及5mg/kg干预组。检测各组小鼠腹腔巨噬细胞中IL-12及脾单个核细胞(PMNC)中Th1类因子IFN-γ、Th2类因子IL-4的表达并分析雷尼替丁作用下IL-12与IFN-γ变化的相关性。结果对照组与哮喘组IL-12分别为(446.93±20.89,211.21±22.42)pg/ml,差异有显著性(P<0.01);IFN-γ分别为(404.91±11.78,271.58±35.35)pg/ml,差异有显著性(P<0.01);而IL-4分别为(20.30±4.93,48.26±9.39)pg/ml,差异有显著性(P<0.01)。雷尼替丁2mg/kg及5mg/kg组IL-12水平分别为(282.64±21.20,354.00±29.97)pg/ml,与哮喘组比较差异有显著性(P<0.01),IFN-γ分别为(313.25±40.54,364.44±39.79)pg/ml,与哮喘组比较差异有显著性(P<0.05,P<0.01),IL-4分别为(37.70±7.47,27.76±9.63)pg/ml,与哮喘组比较有显著性(P<0.05,P<0.01)。雷尼替丁5mg/kg组细胞因子水平改变较2mg/kg组更明显(P<0.01)。在雷尼替丁组,IL-12水平与IFN-γ成明显正相关(r=0.80,P<0.01)。结论雷尼替丁可增加哮喘小鼠体内巨噬细胞IL-12的分泌,并部分纠正Th1/Th2功能平衡,其作用呈剂量相关性。推测对IL-12的上调作用可能是其影响Th1/Th2功能平衡的重要途径。
Objective To observe the influence of ranitidine on the level of Th1/Th2 eytokine expression in asthmatic mice. Methods 40 BALB/C mice were divided into 4 groups,ie, normal eontrol,asthmatie,ranitidine 2mg/kg and 5mg/kg group. Contents of IL-12 in maemphages and concentrations of IFN-γ and IL-4 in spleen mononuelear cells were measured by ELISA,wheras the correlation between IL-12 and IFN-γ in ranitidine groups was analysed. Results Compared with the control group,the levels of IL-12 and IFN-γ were significantly decreased while IL-4 was increased in the asthmatic groups. Compared with the asthmatic group （IL-12：211.21 ± 22.42,IFN-γ：271.58 ± 35.35, IL-4：48.26 ± 9.39）, ranitidine at the dose of 2mg/kg or 5mg/kg groups elevated the levels of IL-12 （282.64 ± 21.20 vs 354.00 ± 29.97, P 〈 0.01 ） and IFN-γ （313.25 ± 40.54 vs 364.44 ± 39.79, P 〈 0.05,0.01 respectively）meanwhile decreased the levels of IL-4 （ 37.70 ± 7.49 vs 27.76 ± 9.63, P 〈 0.05, P 〈 0.01, respectively ）. Furthermore the impact of ranitidine was more obvious in 5mg/kg group than in 2mg/kg group （ P 〈 0.01 ）. It was also demonstrated a positive correlation between IL-12 and IFN-γ in ranitidine groups [ γ = 0.80, P 〈 0.01 ）. Conclusion Ranitidine can up-regulate the production of IL-12 in macrophage in a dose-dependent manner and partly restore the functional balance of Th1/Th2 cytokine expression in asthmatic mice, in which IL-12 seems to be a central factor.
出处
《四川医学》
CAS
2005年第9期940-942,共3页
Sichuan Medical Journal
