摘要
目的研究双基质免疫荧光法检测抗核抗体(ANA)在风湿病中的应用价值。方法采用以喉癌上皮细胞和猴肝细胞为底物的间接免疫荧光法检测211例弥漫性结缔组织病患者(CTD)和43例其他风湿病患者的血清ANA。结果CTD中ANA的阳性率为84.1%,显著高于非CTD风湿病组的2.3%(P<0.01)。ANA阳性者可呈现不同的荧光模式。结论ANA是区别CTD与非CTD风湿病的重要指标,不同的荧光模式有不同的意义。
Objective To study the significance of ANA detected by double substrates immunofluorescence assay. Methods Hep-2 cells and monkey liver tissue were compared as substrates for detection of ANA in serum in 211 pations with CTD and 43 non-CTD subjects, using indirect immunofluoresence assay. Results The positive rate of ANA in CTD group was 84.1%, in non-CTD group was 2.3 %, there was significant difference（P 〈 0.01）. There were different fluorescence models of ANA in the positive objects. Conclusion ANA detected by double substrates immunofluorescence assay is an important method to identify CTD and non-CTD. Different fluorescence models have different significance.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2005年第4期624-626,共3页
Suzhou University Journal of Medical Science
关键词
抗核抗体
结缔组织病
免疫荧光
ANA
connected tissue disease
immunofluorescence
