摘要
【目的】探讨PTEN、p53、细胞核分子抗原(PCNA)在大肠癌的表达及其临床意义。【方法】应用SP免疫组织化学染色检测78例结直肠癌及其癌旁组织和12例正常结直肠黏膜组织中PTEN、p53、PCNA的表达。【结果】PTEN在正常结直肠黏膜组织、癌旁组织、结直肠癌组织表达阳性率分别为100%、96%、50%;p53在正常结直肠黏膜组织中无表达,在癌旁组织、结直肠癌组织表达阳性率分别是2%6、2%;PCNA在正常结直肠黏膜组织中无表达,在癌旁组织、结直肠癌组织表达阳性率分别为3%、73%。PTEN和p53在结直肠癌组织中表达呈负相关(r=-0.610,P=0.000),PTEN和PCNA在结直肠癌组织中表达无相关(r=?0.145,P=0.207)。p53和PCNA在结直肠癌组织中表达呈正相关(r=0.370,P=0.001)。【结论】PTEN失表达,p53基因突变和PCNA过表达均参与结直肠癌的发生,联合检测PTEN、p53和PCNA可为结直肠癌预后判断、随访提供参考依据,具有一定临床价值。
[Objective]To investigate the expression of PTEN, p53 and PCNA proteins in colorectal carcinoma and its significance. [Methods]Expressions of PTEN, p53 and PCNA were detected in paraffin-embed- ded tissues by immunohistochemical technique sp. The specimens included 12 normal colorectal tissues and 78 colorectal carcinoma tissues and surrounding non-tumor colorectal tissues. [Results]Expressions of PTEN in the normal colorectal tissue, the surrounding non-tumor tissue and the colorectal carcinoma tissue were 100%, 96%, 50% respectively.. Expressions of p53 in the surrounding non-tumor tissue and the colorectal cancer tissue were 2%, 62% respectively. Expressions of PCNA in the surrounding non-tumor tissue and the colorectal cancer tissue were 3%, 76%. A positive correlation was noted between expression of p53 and PCNA （ r =0. 370, P〈0.05）, and an inverse correlation was noted between expression of PTEN and p53 in colorectal carcinoma （ r =-0. 610, P 〈0.05）. [Conclusion]Down regulation of PTEN expression, p53 mutation and over expression of PCNA may play a role in occurrence of colorectal carcinoma. Associating PTEN with p53 and PCNA can offer a valuable reference to treatment and follow-up for patients after colorectal carcinoma resection.
出处
《医学临床研究》
CAS
2005年第9期1227-1229,共3页
Journal of Clinical Research
关键词
结肠肿瘤
直肠肿瘤
蛋白质P53
增殖细胞核抗原
colonic neoplasms
rectal neoplasms
protein p53
proliferating cell nuclear antigen
