摘要
【目的】对脐血和外周血树突状细胞(DC)体外扩增进行比较,探索更好的DC来源。【方法】取正常人脐血及外周血,以Ficoll分离提取白细胞,去除悬浮细胞后,分别加入含有GM-CSF、TNF-α及GM-CSF、IL-4的完全培养基中培养,于4、8、10 d进行表型(CD1α、CD83、CD80、HLA-DR)分析及形态观察,1周左右分别检测同种异体混合淋巴细胞反应培养,比较诱导细胞增殖的能力。【结果】脐血诱导的DC细胞数明显大于外周血来源的DC细胞数(脐血2.05±0.05,外周血0.45±0.01),两组有显著差异(P<0.05)。比较两种来源的DC在形态及细胞表型上无显著差异(P>0.05),两者刺激同种异体混合淋巴细胞增殖的能力无显著差异。【结论】脐血和外周血均可成功诱导成熟DC,脐血诱导DC增殖比外周血效率高。
[Objeetive]To compare the peripheral blood and cord blood dendritic cells（DC） by proliferation in vitro in order to get a more accessible source of DC. [Methods]Leukocytes were isolated with Ficoll diatrizoate gradients from normal peripheral blood and cord blood. Cells were washed and then incubated with complete culture system with GM-CSF , TNF-α and IL-4. Detecting phenot ype （ CD1α, CD83, CD80, HLA-DR） and morphology observation on the 4th,8th,10th day, and comparing their ability to activate allo-MLR were made. [Results]Cellular number of DC from cord blood increased over peripheral blood（cord blood 2.05±0.05, peripheral blood 0.45±0.01, P 〈.0.05）, there was significant difference between the two groups. No differences of cell morphology and phenotype were found from the peripheral blood and cord blood DC（67. 2±3. 6）% vs （69. 3±6.8）% for CD1α, （73.7±5.5）% vs （75.6±4.9）% for CD83 ,at 10th day （P〉0.05）. The proliferation ability of DCs from two groups in allogenie mixed lymphocyte reaction （AMLR） was of no significant difference （ P 〉0.05）. [Conclusion]The mature DC is successfully induced from human cord blood and peripheral blood. In particular the cellular number of DCs from cord blood significantly increases over peripheral blood.
出处
《医学临床研究》
CAS
2005年第9期1216-1217,1221,共3页
Journal of Clinical Research
关键词
树突细胞
基因扩增
dendritic cells
gene amplification
