摘要
[目的]探讨熊果酸(ursoIic acid,UA)抑制人胃癌细胞SGC7901增殖与抑制环氧合酶2(cyclooxygenase-2,COX-2)表达间联系.[方法]体外常规培养人胃癌细胞株SGC7901,MTT法检测0、10、20、30、40 μmol/L UA作用不同时间对细胞增殖的影响;倒置显微镜观察不同浓度UA作用24 h后细胞形态变化;Western blot法检测COX-2蛋白表达;放射免疫分析法测定COX-2催化产物前列腺素E2(prostaglandin E2,PGE2)水平.[结果]20～40 μmol/L UA可抑制SGC7901细胞的增殖,并呈浓度和时间依赖性,其作用12、24、36、48 h的药物半数抑制浓度(IC50)分别为(57.50±1.18)、(34.28±2.05)、(27.54±1.11)、(24.83±1.02)μmol/L;20～40 μmol/L UA作用24 h后,SGC7901细胞变圆,出现不同程度的漂浮;COX-2蛋白表达减弱;PGE2水平下降.[结论]熊果酸对SGC7901细胞具有增殖抑制作用,其机制可能与抑制COX-2表达进而减少PGE2生成有关.
[Objective]To investigate the relation between inhibitory effect of ursolic acid（UA） on human gastric cancer cell line SGC7901 and cyclooxygenase-2（COX-2） expression, [Methods]SGC7901 cells were cultured in vitro, MTT assay was used to observe the influence of UA on proliferation of SGC7901 cells after 0, 10, 20, 30, 40 μmol/L UA treatment for different times. After SGC7901 cells were treated by 0-40μmol/L UA for 24 h, morphological changes were observed by inverted microscope. Expression of COX-2 was determined by western blot. The level of PGE2 was detected by radioimmunoassay（RIA）. [Results]20-40μmol/L UA could inhibit the proliferation of SGC7901 cells in a dosage and time-dependent manner, the IC50 value of UA for SGC7901 cells for 12, 24, 36, 48 h was （57.50±1.18）, （34.28±2.05）, （27.54±1.11）, （24.83±1.02） μmol/L respectively; After 20-40μmol/L UA treatment for 24 h, SGC7901 cells turned round and floated. An obvious decline in expression of COX-2 protein was found, and PGE2 level decreased. [Conclusion] UA could inhibit proliferation of SGC7901 cells, which may be caused by COX-2 inhibition and reduced production of PGF2.
出处
《医学临床研究》
CAS
2005年第9期1203-1206,共4页
Journal of Clinical Research
基金
湖北省教育厅基金资助项目(2001A14012)
关键词
胃肿瘤
熊果酸
氧化还原酶类
stomach neoplasms
URSOLIC ACID
oxidoreductases
