摘要
目的在整体动物水平研究淫羊藿总黄酮(EF)延缓衰老的效果和分子机制。方法采用果蝇寿命实验,观察EF延长果蝇寿命的效果;采用果蝇全基因组寡核苷酸芯片研究EF延缓衰老的机制。结果空白对照组、V it E组、EF低剂量组及高剂量组平均寿命分别为(34.90±23.04),(35.25±23.41),(41.48±25.00)和(46.23±26.20)d,高低剂量EF均显著延长果蝇的平均寿命(P<0.01或P<0.05),高剂量EF提高平均寿命32.46%。老年果蝇呼吸链相关酶、蛋白分解酶、促凋亡基因上调2倍以上,而自由基清除酶、抗凋亡基因、内分泌免疫相关基因下调2倍以上。EF能下调老年果蝇呼吸链相关酶而上调自由基清除酶基因,下调蛋白分解酶基因,能上调促进细胞周期、抗凋亡、免疫相关基因。结论老年果蝇存在自由基产生过多而清除减少、凋亡加速、蛋白溶解过度、内分泌免疫功能低下的内在机制,EF通过减少自由基的产生,增加自由基的清除,减少蛋白分解,促进细胞增殖,恢复免疫功能、对抗凋亡而发挥延缓衰老的功效。
Objective To investigate senescence-delaying efficacy of epimedium flavonoids （EF） and the underlying mechanisms in model organism drosophila. Methods The effect of EF on longevity of drosophila was detected by lifespan test, and the mechanisms were probed using oligonucleotide microarray. Results Average longevity in control, Vit E group,low- and high-dose EF group was 34.90 ± 23. 04,35.25 ± 23.41,41.48 ± 25.00,46.23 ±26.20 days,respectively. The longevity was increased by high-dose EF by 32.46% （P 〈 0.01 or P 〈 0.05）. The old drosophila displayed up-regulation of respiratory chain related enzymes, proteases, pro-apoptotic genes by 2-fold or more. While enzymes responsible for removal of oxygen free radicals,anti-apoptotic genes,endocrine and immunity related genes were down- regulated by 2-fold or more. After treatment by EF,respiratory chain related enzymes and proteolysis enzymes were decreased and enzymes responsible for removal of oxygen free radicals, cell cycle promoting genes, anti-apoptic genes and immunity related genes were increased. Conclusions Senile drosophila have inherence mechanism of excessive production of free radicals and less decreased removal of it, excessive apoptosis,proteolysis and deficiency of immunity. EF can decrease production and increase removal of free radicals,promote cell proliferation.recover immune function and withstand apoptosis to achieve senescence-delaying efficacy.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2005年第9期1061-1063,共3页
Chinese Journal of Gerontology
关键词
淫羊藿总黄酮
衰老
基因表达谱
自由基
果蝇
Epimedium flavonoids
Senescence
Gene expression profiling
Oxygen free radicals
Drosophila
