摘要
目的建立二级质谱与高效液相色谱联用的方法测定人血浆中贝那普利及其活性代谢产物贝那普利拉浓度的新方法。方法色谱柱为ZorbaxC18(5μm,4.6mm×150mm)。流动相为乙腈-10mmol/L乙酸胺(含0.08%NH3)(70∶30,v/v),流速1mL/min,柱后分流4∶1。样品经乙腈沉淀蛋白后直接进样。用选择离子分别对贝那普利、贝那普利拉和内标依拉普利进行检测。结果测定贝那普利及贝那普利拉的线性范围均为0.5～600μg/L,最低检测浓度为0.1μg/L。日内精密度<2.6%,日间精密度<3.5%。方法回收率范围为100.9%～110.2%。结论本方法精密、准确,适用于贝那普利及贝那普利拉的药物动力学及生物等效性研究。
[Objective] To constitute a HPLC-MS/MS method to determine serum benazepril and it active metabolites benazeprilate. [Method] Using Zorbax C18 Column and acetonitrile-10mM acetic ammonia （70：30, contain 0.08% ammonia） as the mobile phase. Blood sample were extract by using acetonitrile, and 20μL were applied to the HPLC system. Using selected iron to detect benazepril, benazeprilate and benapril respectively.[Result] The detective limited concentration is 0.1μg/L. The linear range of benazepril and benazeprilate are both in 0.5～600 μg/L. The within day RSD and between day RSD was 2.6% and 3.5% respectively in this linear range. Method recovery is within 100.9% to 110.2%. [Conclusion] The method is accuracy and sensitive enough to study on the Pharmacokinetics of benazepril and benazeprilate.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第17期2630-2631,2635,共3页
China Journal of Modern Medicine
