摘要
目的检测趋化因子IP-10和MIP-3α在肝移植大鼠肝脏中的表达来研究两者在肝移植免疫中的作用。方法建立大鼠肝移植模型,在移植后不同的时间点(3、5、7、10d)取肝脏标本,用免疫组织化学法检测IP-10和MIP-3α在肝组织的表达。结果肝急性排斥组与对照组相比,组织坏死明显。汇管区及肝索的浸润细胞,部分浸润细胞包绕的肝细胞、胆管上皮、小叶间静脉内皮以及Kupffer细胞均可见IP-10的表达。MIP-3α在肝脏的表达主要是浸润的淋巴细胞和Kupffer细胞。两者的表达均于移植后第5天达高峰,两组间表达差异有显著性。结论IP-10在肝组织细胞的表达导致了活性T细胞的杀伤,从而造成组织坏死;MIP-3α可招募未成熟DC进入移植物,为活化T细胞进而诱发特异性免疫应答做准备。
[Objective] To explore the effects of IP-10 and MIP-3 in rat liver allograft immune response, we detect the expression of IP-10 and MIP-3 in liver at different times postoperatively. [Methods] Different rat allograft models were established. Acute liver allograft rejection groups： Wistar hver to SD rats; Control groups： SD liver→SD rats. Liver samples were collected on days 3, 5, 7,10 postoperatively （each n=3）. The expressions of IP-10 and MIP- 3 in hver were examined by means of immunohistochemistry. [Results] Compared with control groups, the change of histology in acute liver allograft rejection groups was serious. The expression of infiltrated cell in liver lobule and necrosis area increased. The expression of IP-10 was mainly focused on infiltrated cells, hepatocytes surrounded by infdtrated ceils, duct endothelial cells, vein endothelial cell and kupffer cells. The expression of MIP-3 was mainly focused on infiltrated ceils and kupffer cells, the expression of IP-10 and MIP-3 reached a peak on days 5 postoperatively and had significantly difference in liver between acute rejection groups and control groups. [Conclusion] The high expression of IP-10 in hepatocytes and nonparenchymal cells may be involved in tissue necrosis induced by activated T lymphocytes. The high expression of MIP-3 α in liver may be related with dendritic cell migrating, which do favor to activate T cell and then induce adaptive immune response.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第17期2594-2596,2601,共4页
China Journal of Modern Medicine
