摘要
建立了红花中的主要功效成分羟基红花黄色素A含量测定的RP-HPLC方法．色谱柱为Betabasic 18柱（250mm×4．6mm，5μm），流动相为甲醇和0．5％乙酸水溶液，梯度洗脱，流速1mL／min，柱温30℃，检测波长为402nm，线性范围：0．0432～0．864μg，检测限2．1ng（按信噪比S／N=3计），该方法快速、准确。
A method for determination of the main effective component hydroxysafflor yellow A in Carthamus tinctorius L. by RP-HPLC was established. The chromatographic column was Betabasic 18, eluted in gradient with a methanol-0.5 % aqueous acetic acid mobile phase, 1.0 mL/min flow rate,30℃ column temperature, and 402 nm detection wavelength. The linear range was from 0.043 2 μg to 0. 864 μg, and the limit of detection was 2.1 ng （ S/ N = 3）. The method was fast and accurate, and gave satisfied results when applied to determine hydroxysafflor yellow A in Carthamus tincctorius L..
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2005年第4期47-49,共3页
Journal of Henan University of Technology：Natural Science Edition
关键词
反相高效液相色谱法
羟基红花黄色素A
红花
reversed phase high performance liquid chromatography （RP-HPLC）
hydroxysafflor yellow A
Carthamus tinctorius L.
