摘要
采用强致病力菌株,制备成棉子菌粉载菌体和孢子悬浮液,研究不同菌量、不同接菌方法和致病温度,诱导棉花苗期抗枯、黄萎病性的育种新技术.通过4种接种剂量梯度试验表明,棉花苗期诱导抗性的最佳接菌量是90 g·m-2棉子菌粉载菌体,播种前土壤接菌,棉黄萎病菌孢子悬浮液的最佳接菌浓度是2×107孢子·ml-1,2片真叶时伤根接菌10 ml,薄膜拱盖提温,20～25℃是诱导发病的最适温度,接菌15～20 d后,淘汰棉枯萎病发病8%以上和棉黄萎病12%以上的品种群体材料,并淘汰抗病群体材料中的感病个体,构建既抗棉枯萎病、又抗棉黄萎病性的多抗病性育种方法.
The strong virulence pathogen strains of wilt diseases were cultured in autoclaved cottonseed to make pathogen carrier respectively. Fusarium oxysporum f. sp. Vasinfectum was inoculated with cottonseed meal at the dosage of 90 g·m^-2 in nursery soil before sowing. Then each seedling was inoculated with 10ml of Verticillium dahliae spore suspension at the concentration of 2 × 10^7 spores per milliliter by watering injured roots at 2 true leaf growth stage. The nursery was covered with polythene film to keep the average bed temperature of 20-25℃. Thus the wilt diseases symptoms would appear on susceptible plant within 15-20 days after inoculation of Verticillium dahliae spore suspension. The varieties, of which Fusarium wilt disease incidence was over 8% or Verticillium wilt was over 12%, and the susceptible plants of resistant variety were eliminated. Then the resistant plants of resistant varieties were selected and transplanted to the field. It had been proved that this method of inducing cotton multi-resistance to wilt diseases by selection with artificial inoculation was effective and practicable. With applying of this method, several cotton resistant varieties had been bred.
出处
《棉花学报》
CSCD
北大核心
2005年第5期264-268,共5页
Cotton Science
基金
国家育种攻关(96-002-02-10-02)
四川省育种攻关(2001-06-01)
国家发展棉花生产专项资金(200012)
关键词
棉花
抗病育种
诱导抗性
cotton
resistance breeding
induced resistance by selection
