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食管癌组织3p、18q位点微卫星杂合性缺失的研究 被引量：3

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摘要 目的探讨食管癌组织中3p14.3～3p21.1、18q22.3～18q23杂合性缺失(loss of heterozygosity, LOH)频率与人食管癌发生及其临床病理特征的关系.方法应用聚合酶链反应(polymerase chain reaction, PCR)和变性聚丙烯酰胺凝胶电泳技术(denaturalized polyacrylamide gel electrophoresis, PAGE),对28例食管癌中微卫星DNA序列不稳定性(microsatellite instability, MSI)表达情况进行研究.结果 28例食管癌标本中23例鳞状细胞癌9例LOH阳性,5例食管小细胞癌4例LOH阳性;LOH与肿瘤的病理分级、PTNM分期、有无区域淋巴结转移和浸润深度无相关性(P>0.05);3P位点上有较高频率的LOH发生.结论 LOH与食管癌的临床病理类型、临床分期、细胞分化程度、癌组织浸润深度和有无区域淋巴结转移等参数无相关性(P>0.05);食管癌在多个染色体位点均存在LOH现象,3p是食管癌相关的热点区域,3p位点基因的改变在食管癌发生过程中具有较重要意义.
作者 翟瑜 杨月卿 苏力 杜权 郭贵军
机构地区 河北省人民医院普外三科 河北省人民医院老年病四科
出处 《河北医科大学学报》 CAS 2005年第5期355-358,共4页 Journal of Hebei Medical University
关键词 食管肿瘤 DNA 杂合性丢失 聚合酶链反应
分类号 R735.1 [医药卫生—肿瘤]
  • 相关文献

参考文献12

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二级参考文献19

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共引文献21

同被引文献40

  • 1翟瑜,苏力,杜权,杨月卿,郭贵军,李保庆,单保恩,王桂琦.食管癌组织3p、18q位点微卫星DNA序列不稳定性的研究[J].诊断学理论与实践,2005,4(2):145-149. 被引量：8
  • 2李卫东,王亮,王秀琴,张春林,张涛,毛学正,吴旻.食管癌组织染色体位点特异性的杂合性丢失和微卫星DNA序列不稳定[J].中华医学遗传学杂志,1996,13(4):194-197. 被引量：17
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引证文献3

二级引证文献1

河北医科大学学报
2005年 第5期

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