摘要
目的探讨白细胞介素-4(interleukin 4,IL-4)和表面活性蛋白A(surfactant protein-A,SP-A)在肺纤维化大鼠中的表达水平及早期诊断意义。方法选取60只Wistar大鼠随机分为正常对照组、博莱霉素致肺纤维化模型组,每组30只,分别于造模后1、3、7、14、28天处死大鼠。应用酶联免疫吸附法(enzyme-linked immunosorbentassay,ELISA)及Western blot测定不同时相点血清IL-4、SP-A水平。结果模型组血清IL-4早期较对照组显著升高(P<0.01),血清SP-A各时相均高于对照组(P<0.05),并随病情进展而逐渐升高。结论血清IL-4、SP-A可作为诊断肺纤维化早期肺泡炎的灵敏指标,SP-A可反映肺纤维化严重程度。
Objective To detect the levels of serum interleukin-4 （IL-4） and surfactant protein-A （SP-A） in rats with pulmonary fibrosis that were induced by bleomycin and to evaluate their significance in early diagnosis of pulmonary fibrosis. Methods Sixty rats were randomly divided into two groups, control group and pulmonary fibrotic group, with 30 rats in each group. The rats were killed in turn at 1, 3, 7, 14 and 28 day. Enzyme-linked immunosorbent assay （ELISA） and Western blot were used to detect the levels of serum IL-4 and SP-A. Results The levels of serum IL-4 of the pulmonary fibrotic group were significantly higher than those of the control group at early stage （P〈0.01）, while the levels of SP-A of the former group were always higher （P〈0.05）. and tended to increase with the development of the disease. Conclusion Serum IL-4 and SP-A may be sensitive indicators in diagnosing early stage pulmonary fibrosis and SP-A reflects the development stages of the disease.
出处
《河北医科大学学报》
CAS
2005年第5期337-339,374,共4页
Journal of Hebei Medical University
