摘要
目的:通过光镜及电镜观察大鼠高眼压视网膜缺血再灌注不同时间点的组织病理学改变。方法:采用前房灌注生理盐水的方法制作实验性视网膜缺血再灌注模型,分别在再灌注不同时间点取材,通过光镜和电镜观察视网膜组织形态学及超微结构改变。结果:缺血再灌注组早期主要表现为内核层水肿增厚,晚期表现为视网膜萎缩变薄,视神经节细胞减少。视网膜组织细胞凋亡见于缺血再灌注12 h2、4 h及48 h组,凋亡细胞主要位于内核层及神经节细胞层。结论:高眼压视网膜缺血再灌注可造成视网膜结构严重损害。
Objective： To observe the morphological changes of retinal iniury in ischemia-reperfusion rats by microscopy and tranmisslon electron microscopy. Methods： The model of experimental ischemia-reperfusion injury was created by increasing the intraocular pressure of the rats. The retinal histopathologic and ultrrastructural morphological changes were observed with the microscope and the transmission electron microscope at different times. Results： The inner retinal layer swelled evidently in the early period and became atrophy;and the account of retinal ganglion cells decreased. The apoptotic cells could be seen at 12,24 and 48 hour after ischenaia, which mainly occurred in the retinal ganglion cells and the inner nuclear layer. Conclusions： Retinal ischemiareperfusion may cause serious damage to histopathology and ultrastructure of the retina.
出处
《蚌埠医学院学报》
CAS
2005年第5期385-387,共3页
Journal of Bengbu Medical College
关键词
视网膜病变
缺血再灌注损伤
凋亡
大鼠
retinal diseases
ischernia-reperfusion injury
apoptosis
rats
