摘要
为了进一步综合研究运动性免疫抑制的机制,并探讨谷氨酰胺(Glutamine,Gln)和/或精氨酸(Arginine,Arg)对调节机体免疫功能的影响及其机制,以及在预防运动性免疫抑制中的交互作用。通过对大鼠进行大负荷的运动训练建立运动性免疫抑制的模型,并补充Gln和/或Arg,测定了训练机体淋巴细胞的增殖功能、细胞因子和淋巴细胞的凋亡率等指标。结果发现:1)经过8周的大负荷运动训练后,大鼠外周血液中淋巴细胞转化率、血清IL-2、Gln和Arg含量显著降低,而淋巴细胞凋亡率和血清sIL-2R含量却显著升高。2)在大负荷运动训练后补充Gln或Arg,能够显著防止大负荷运动训练后血清中Gln和Arg含量的降低,使大鼠外周血中淋巴细胞的转化率和血清IL-2含量显著升高,并使淋巴细胞凋亡率和血清sIL-2R含量显著降低。3)联合使用Gln和Arg,虽然能提高大负荷运动训练后大鼠外周血淋巴细胞对PHA的增殖效应和血清IL-2含量,但是并没有明显的交互作用。但是对淋巴细胞的凋亡率和血清sIL-2R含量具有显著的交互作用,使外周血中淋巴细胞凋亡率和血清sIL-2R含量进一步降低。
In order to explore the mechanism of exercise-induced immunosuppression, and the effects and mechanism of Gin and/or Arg on modulation of immunologic function, and their integrated effects on sports Sports immunosuppressive model was established successfully through SD rats were conducted heavy load swimming training for 8 weeks. Meanwhile the rats were administrated for Gin and/or Arg. The immunologic indexes including lymphocytic proliferation function, serum cytokine, glutamine and arginine concentrations, and apoptosis of lymphocyte in peripheral blood were measured. The results showed： 1） After heavy training for 8 weeks, the serum IL-2, Gin, Arg concentrations and lymphocytic proliferation function decreased significantly, but the serum sIL-2 concentration and rate Of lymphocytic apoptosis increased drastically. 2） Supplement of Gin or Arg after heavy training might prevent the decrease of serum Gin and Arg concentration, and increase the IL-2 concentration and lymphocytic proliferation function markedly. In the meantime, and decrease the serum sIL-2 content and the rate of lymphocytic apoptosis significantly. 3） Combinative supplement of Gin and Arg after heavy training have significant interaction on serum sIL-2R content and the rate of lymphocytic apoptosis. Although it may further increase serum IL-2 and lymphocytic proliferation function, there was no significant interaction.
出处
《北京体育大学学报》
CSSCI
北大核心
2005年第8期1075-1077,共3页
Journal of Beijing Sport University
基金
江苏省教育厅自然科学基金资助项目E0109188
国家科技部科技攻关课题2001BA904b17
北京体育大学博士论文
