摘要
目的研究高糖对人腹膜间皮细胞(HPMC)纤维连接蛋白(FN)及纤溶酶原激活物抑制剂-1(PAI-1)的影响。方法利用HPMC体外培养模型,HPMC分别经含1.5%、2.5%、4.25%葡萄糖的1640培养基培养6、12、24、48h后,用ELISA法检测HPMC分泌FN及PAI-1的情况。结果11.5%、2.5%、4.25%葡萄糖作用于HPMC,使培养上清中的FN明显高于对照组(P<0.05),且FN增高的程度与葡萄糖浓度和作用时间呈依赖关系。22.5%、4.25%的葡萄糖作用于HPMC,使培养上清中PAI-1明显高于对照组(P<0.05),且增高的程度与葡萄糖浓度和作用时间呈依赖关系,1.5%葡萄糖液与对照相比没有差别(P>0.05)。结论高糖促使HPMC分泌FN、PAI-1增加,在腹膜纤维化的进程中起一定作用。
Objective To evaluate the effect of high glucose on the expression of fibronectin （FN） and plasminogen activator inhibitor-1 （PAI-1） in human peritoneal mesothelial celIs（HPMC）. Methods The cultured HPMC were exposed to the culture medium containing different concentrations of glucose （1.5 %, 2. 5 %, 4. 25 % ） for 6,12,24, 48 hours, and normal culture medium was used as control. FN and PAI-1 in supernatant were detected by enzyme-linked immunoassay（ELISA）. Results The glucose at 1.5%,2. 5%,4. 25% resulted in a time-and concentration-dependent increase in FN expression in HPMC （P〈0. 05）. The glucose at 2.5%, 4. 25 % increased PAI-1 expression in HPMC（P〈0. 05）, and the effect was time- and concentration-dependent. PAI-1 expression was not affected by 1.5% glucose（P〈0. 05）. Conclusion High glucose increases the expression of FN and PAI-1 in HPMC, thus playing a part in the progression of peritoneal fibrosis.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2005年第5期700-702,共3页
Journal of Sichuan University(Medical Sciences)
