摘要
初步研究了MycobacteriumE3的生长和产酶特性，利用MycobacteriumE3休止细胞催化烷烃羟化和烯烃环氧化．研究结果表明，烷烃不能被羟化，烯烃环氧化具有底物选择性．对烯丙基型底物XCH2CH＝CH2（X＝H、Cl、Br、OH），取代基大小显著影响环氧化活性Mycobac-teriumE3中烯烃单加氧酶和另一种结构未知的酶的存在导致烯烃环氧化过程中存在环氧化物的立体选择性形成和非立体选择性降解两种竞争反应，旋光技术和手性气相色谱揭示MycobacteriumE3催化相应烯烃主要形成（R）-1，2-环氧丙烷和（S）-1-氯-2，3-环氧丙烷。
Growth Characteristics of Mycobacterium E3 on ethene in mineral salts mediumand oxidation of alkanes and dsenes catalyzed by Mycobacterium E3 resting cell werestudied. Mycobacteriurn E3 was unable to, catalyze hydroxylation of alkanes, but ableto catalyze epoxidation of alkenes specifically. Influence of substituents on epoxidizingactivity was discussed for allyl substrates XCH2CH=CH2 (X=H, Cl, Br,OH). Alkenemonooxygenase and an unknown enzyme in Mycobacterium E3 catalyzed stereoselectiveepoxidation of alkenes and not-stereoselective degradation of epoaldes. Optical rotationand chiral capillary chromatography revealed that propene and 3-chloro-1-propene wereepoaldized to (R)-1,2-epoxypropane and (S)-1-chloro-2,3-epoxypropane.
出处
《物理化学学报》
SCIE
CAS
CSCD
北大核心
1996年第3期218-223,共6页
Acta Physico-Chimica Sinica
基金
国家自然科学基金
关键词
烯烃单加氧酶
烯烃
环氧化
催化反应
Mycobacterium E3, Alkene monooxygenase,Epokidation of alkenes,Stereoselectivity
