风湿性心脏病心肌中核转录因子的表达及其意义被引量：9

Expression and significance of nuclear transcription factor in myocardium of rheumatic heart disease

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摘要目的了解风湿性心脏病患者心肌中核转录因子表达的特点,探讨核转录因子与风湿性心脏病心肌纤维化的关系。方法风湿性心脏病患者30例,术中取左室乳头肌,以成年正常乳头肌10例为对照,苏木素-伊红(HE)、Masson染色检测心肌胶原,免疫组织化学方法观察核因子-kappa B(NF-κB)、活化蛋白-1(AP-1)在心肌中的表达,均用图像分析仪定量分析。结果风湿性心脏病心肌组织中间质胶原纤维明显增生。NF-κB与AP-1在风湿性心脏病心肌中阳性表达增强,表达强度与心肌纤维化面积呈正相关(NF-κB:r=0.8945,AP-1:r=0.9011,P<0.01),与NYHA心脏功能分级呈正相关(NF-κB:r=0.7247,AP-1:r=0.7879,P<0.01);在正常心肌中表达呈弱阳性或阴性。结论NF-κB与AP-1均在风湿性心脏病心肌纤维化形成中起作用。 Objective To investigate the effects of nuclear transcription factor-kappaB(NF-kB)and activated protein-1(AP-1)on rhcumatic myocardial fibrosis.Methods Thirty rheumatic myocardial specimens and 10 normal samples were examined by Masson's staining.immunobistochemical staining adn image analysis.Results Both NF-kB and AP-1 were expressed in the nucler of myocardium with rheumatic heart disease(RHD).The degree of positive staining in myocardiurn with RHD was significantly correlated with the cardial collagen volume(NF-kB:r=0.8945,AP-1:r=0.9011,P〈0.01)and the NYHA functional classes(NF-kB:r=0.7247,AP-1:r=0.,7879,P〈0.01).Conclusion Both NF-kB and AP-1 may play a key role in rheumatic myocarlial fibrosis.so a new thcrapeutic strategy to prevent or regress myocardial fibrosis at molecular level may be found.

作者任崇雷刘维永张近宝 REN Chong-ei;LIU Wei-yong;ZHANG Jin-bao(Department of Cardiorascular Surgery,The 466th Hospital of PLA.Beijing 100089,China)

机构地区解放军第四六六医院心血管外科第四军医大学西京医院心血管外科

出处《中华风湿病学杂志》 CAS CSCD 2005年第9期534-536,i0001,共4页 Chinese Journal of Rheumatology

基金国家自然科学基金资助项目(39870790)

关键词风湿性心脏病心肌细胞核转录因子基因表达心肌纤维化胶原纤维 Rheumatie heart disease Myocardliuny Collagen NF-kappaB Transcripition factor AP-1

分类号 R541.2 [医药卫生—心血管疾病]

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