摘要
通过考虑溶质和溶剂在液相色谱体系中的相互作用，从理论上给出了一个描述溶质在液相色谱体系中进样量与保留值之间关系的方程。由方程可以证明，当进样量趋于零时，溶质的保留值为一定值，当进样量无限大时，溶质的保留值趋于零，且随着进样量的增加，溶质的保留值必然减小。通过方程的线性形式，可以获得两个描述色谱体系特征的重要参数：一个是溶质与固定相相互作用的平衡常数Ｋ，另一个是假想的分布在固定相表面上的活性点总数。用一些蛋白质的疏水色谱体系的实验结果对方程进行了检验，结果表明，实验保留值与方程的预测值符合得较好，相对误差在５％以内。
n equation describing the reistionship between the injected amount of solutes and their retention values in liquid chromatography was theoretically presented by considering the interaction between the solute molecules and the solvent molecules on the surface of stotionary phase.It can be proven that when the injected amount of solutes separately approach to infinity or to zero, their retention values separately approach to zero or to a constant depending on the chromatographic system, and when the injected amount of solute increases from zero to infinity, the retention value will constantly decrease. By using the linear form of this equation, two important parameters describing the characteristics of the chromatographic system can be determined. One is the thermodynamic equilibrium constant K for solute absorption on the surface of the stationary phase and another is the total mean active sites hypothetically distributed on the surface of stationary phase. Some proteins were used to test this equation in hydrophobic interaction chromatography, and it was found that the predicted retention values by using this equation are very consistent with the experimental ones, the deviations between them are less than 5%.
出处
《色谱》
CAS
CSCD
北大核心
1996年第3期179-181,共3页
Chinese Journal of Chromatography
基金
"863"生物领域资助
关键词
液相色谱法
进样量
保留值
liquid chromatography, amount of injected solute, retention factor
