摘要
经分子设计改造的酵母转录活化因子Ａｃ─［Ｑ^（２３６），Ｑ^（２４２）］ＧＣＮ４（２２６—２５２）—ＧＧＣ—ＮＨ_２的化学合成及二聚化张若衡，王鹤，徐筱杰，唐有棋（北京大学物理化学研究所，北京，１００８７１）关键词酵母转录活化因子，固相肽合成，空气氧化...
Yeast transcriptional activator GCN4(226-252)-GGC-NH_2 and the designed Ac-[Q￣(236),Q￣(242)]GCN4(226-252)-GGC-NH_2 aiming at altering GCN_4 DNA-binding specificity was prepared by stepwise solid phase peptide synthesis.It was found that the yield of the peptide would be greatly improved when treated with dithiothreitol(DTT).The dimer was obtained by air oxidation, which would be used in the sequence-specific DNA binding activity experiments.
出处
《高等学校化学学报》
CSCD
北大核心
1996年第5期747-749,共3页
Chemical Journal of Chinese Universities
基金
国家"攀登计划"项目
关键词
酵母转录
活化因子
二聚体
亮氨酸
Yeast transcriptional activator
Solid phase peptide synthesis
Air oxidation
