摘要
以芽孢杆菌为尿酸氧化反应的酶源，采用氧电极为基础电极，制成尿酸微生物传感器。对细菌的诱导培养条件、电极的工作性能、电极再生以及微生物酶反应动力学行为进行了研究。在ｐＨ＝８．５时，电极的线性范围为１．０×１０￣（－５）～４．０×１０￣（－４）ｍｏｌ／Ｌ，检出下限为１．０×１０￣（－６）ｍｏｌ／Ｌ，测得微生物膜中尿酸酶的表观米氏常数为２．８×１０￣（－４）ｍｏｌ／ｌ，酶反应活化能为４７０００Ｊ／ｍｏｌ，温度系数为１．８．
An electrode for the determination of uric acid was prepared. It employed a kind of bacillus as the biocatalyst and an oxygen gas electrode as the basic electrode, The effect of the culture medium, the response character of the electrode and its kinetic mechanism are studied. At suitable pH, the uric acid concentration is linear in the range of 1. 0×10￣(-5)～4.0 ×10￣(-4)mol/L, the apparent Michaelis constant K_m is 2. 8×10￣(-4) mol/L, the activation energy is 47 000J/mol, and the temperature coefficient is 1. 8.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1996年第5期702-706,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
中国科学院长春应用化学研究所电分析化学开放实验室资助
