摘要
由于非洲爪蟾卵母细胞体积较大,故其蛋白质和RNA合成活性很高。同时,卵母细胞本身仅有很少几种内源性通道和受体,因此可以忠实、高效地表达多种类型的外源性通道和受体。非洲爪蟾卵母细胞现已成为一种研究外源性离子通道基因的工具,在分析多亚基通道翻译后的加工和组装、检测突变通道特性、比较不同组织来源的通道特性、检测第二信使系统对通道功能的调制、分析和鉴定编码通道蛋白的克隆基因等方面有着广泛的应用。
Xenopus laevis oocytes have been extensively employed to investigate the structure and function of exogenous ion channel. This paper briefly reviews the application of Xenopus laevis oocytes in researches on reconstitution of muhi-subunit channels, characteristics of mutation channel, properties of the same type channel from different tissue, modulation of the second messenger system on channel function, and cloned ionic channels genes. Their applications provide the basis for studying the relationship between structure and function of channels, and further exploring the mechanism of disease associated with ion channel mutation.
出处
《武汉科技大学学报》
CAS
2005年第3期302-305,共4页
Journal of Wuhan University of Science and Technology
关键词
爪蟾
卵母细胞
离子通道
基因表达
Xenopus laevis oocytes
ion channels
gene expression
