摘要
目的探讨重组白细胞介素-2(IL-2)和γ-干扰素(IFN-γ)对日本血吸虫小鼠肝纤维化作用. 方法 ABC免疫组化法,VG染色及多媒体图文分析,观察不同时期感染血吸虫小鼠肝脏IL-2、IFN-γ和胶原纤维变化以及分别注射重组IL-2、IFN-γ后小鼠肝脏IL-2、IFN-γ和胶原纤维变化. 结果小鼠肝脏IL-2、IFN-γ水平随感染时间延长而缓慢下降,胶原纤维含量随感染时间延长逐渐上升;注射重组IL-2或IFN-γ组小鼠随着相应因子补充显著上升,胶原纤维含量逐渐下降,末次注射后1～6周,肝脏相应因子水平明显高于正常组和感染组P<0.01,胶原纤维含量明显低于感染组P<0.01. 结论重组IL-2和IFN-γ明显降低肝脏胶原纤维合成,延缓肝纤维化发生.
OBJECTIVE To detect the expression of IL-2 and IFN-γ in the liver at different period postinfection of Schistosoma japonicum and their effect on liver fibrosis after supplementary injection of these cytokines. METHODS The ABC immunohistochemistry and pathologic image multimedia quantification system were applied to detect activity of IL-2 and IFN-γ. The VG dye was applied to detect collagen of liver. RESULTS The level of IL-2 and IFN-γ in the liver in infected but untreated group decreased slowly （from 8, 12, 15 to 18 weeks） , the level of collagen in the liver in this group increased slowly （P〈0.01）. The supplementary injection of the cytokines at wk 15 postinfection in the treated groups increased the cytokines significantly, the level of IL-2 and IFN-γ was higher at wks 1-6 after the last injection than that of infected but untreated group, the level of collagen in the liver in this group was lower （P〈0.01）. CONCLUSIONS Recombinant interleukin-2 and interferon-γ markedly inhibit the synthesization of collagen in the liver, corresponding to a diminished degree of the liver fibrosis.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2005年第9期981-983,共3页
Chinese Journal of Nosocomiology
基金
湖北省血吸虫病防治委员会基金资助(2001-2)
关键词
血吸虫病
日本
重组IL-2
重组IFN-γ
胶原纤维
Scshistosomiasis japonica
Recombinant interleukin-2
Recombinant interferon-γ Collagen
