摘要
目的:观察经诱导的胚胎干细胞(ES)移植在小鼠脊髓损伤和小鼠缺氧缺血性脑病中的存活和迁移。方法:利用小鼠脊髓损伤(SCI)和缺氧缺血性脑病的模型(HIE),将小鼠胚胎干细胞体外诱导分化为ES细胞的衍生细胞后,注射到脊髓损伤和缺氧缺血性脑病的小鼠体内。移植后观察12周,应用酶学的方法检测Lac-Z标记的经诱导的ES细胞在脊髓和脑内的存活和迁移情况。结果:ES细胞在中枢神经系统损伤区能存活,并进行长距离的迁移,并与周围组织整合。结论:经诱导的胚胎干细胞能在宿主损伤脑和脊髓中存活、迁移,且脑内迁移较脊髓内迁移明显。
Objective： To observe the survival and migration bv transplantation of embryonic stem cells in SCI and HIE in mouse, Method： Labeled XY with Lac-Z marked gene, of mouse ES cell was cultured and induced In differentiate by specialized factors in vitro. ES cell-derived cells were planted into vertebral canal around injured spinal cord and cerebral ventricle of HIE model. Model animals were observe at 8 weeks after transplantation. Spinal cord and brain tissue were harvested for enzymology staining to detect whether the Lac-Z- labeled gene ESc express. Result：ES cell-derived cells could survive and migrate in the injured region of central nervous system in a long distance, and communications with peritissue.Conclusion：ES cell-derived cells couhl surive and migrate in central nervous system injured region, brian migration was more apparent in comparision with spinal migration.
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2005年第8期569-571,i0001,共4页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金资助(30470599/C030307)
关键词
中枢神经系统损伤
胚胎干细胞
移植
小鼠
central nervous system injury
embryonic stem cells
transplantation
mouse
