摘要
目的:了解白细胞介素10(IL-10)在系统性尖端赛多孢子菌感染中的表达情况。方法:建立小鼠系统性尖端赛多孢子菌感染模型,包括致死量感染组(健康鼠5×106孢子尾静脉接种)、亚致死量感染组(健康鼠5×105孢子尾静脉接种)及免疫抑制组(地塞米松诱导免疫抑制鼠给予5×105孢子尾静脉接种),用逆转录—聚合酶链反应技术(RT-PCR)检测脾内白细胞介素10(IL-10)mRNA水平,用平皿系列稀释法检测肾内菌落形成单位(CFU),并记录平均生存时间(MST)。结果:致死量组中小鼠脾内IL-10表达明显上升(3 d,P<0.01;7 d,P<0.01),小鼠肾内大量菌生长,且7 d高于3 d(P<0.01),小鼠平均13.6 d均死亡;亚致死量感染组中,IL-10表达基本正常,肾内菌量少,且7 d低于3 d(P<0.01),小鼠生存时间均超过45d;与正常对照组相比,免疫抑制组小鼠脾内IL-10表达明显上升(3 d,P<0.01;7 d,P<0.01),小鼠肾内大量菌生长,且7 d高于3 d(P<0.01),小鼠平均14.2 d死亡,此外,免疫抑制组小鼠脾内IL-10及肾内菌量均高于相应时间亚致死量组(3 dP<0.01,7 dP<0.01:CFUs:3 dP<0.01,7 dP<0.01)。结论:IL-10在小鼠系统性尖端赛多孢子菌感染中表达增高,可能起着促进疾病发展的作用。
Objective ： To investigate the role of lnterleukin - 10（ IL - 10） in experimental murine of systemic scedosperium apiospermum infection. Methods： Three murine systemic scedosperium apiospermum infection models were created, including lethal doses group （5 × 10^6 spores were inoculated in intact mice by tail vein） , sublethal doses group（ 5 × 10^5 spores were inoculated in intact mice by tail vein） and immunosuppressed group （ dexamethasone - induced immunosuppressed mice were inoculated with 5 × 10^5spores by tail vein）. In these models, reverse transcription- polymerance chain reaction technique（ RT- PCR） was applied to detect the expression of mRNA for IL - 10 in spleens, clone forming units （CFU） of fungi in the infected kidneys were determined with the plating dilution method, and mean survival time（MST） of the mice was also recorded. Results： In lethal doses group, the expression of IL - 10 mRNA increased markedly（3 d, P 〈0.01; 7 d, P 〈0. 01 ）. There were a large amount of fungi in kindeys of lethal doses group and CFUs in kidneys on 7 days were higher significantly than on 3 days post - infected （P 〈 0. 01 ）. The MST was 13.6 days for lethal infection group. In the sublethal doses group, the expression of mRNA for IL - 10 was within normal limits. There were a few fungi were found in kindeys of sublethal doses group and CFUs in kidneys on 7 days were lower significantly than on 3 days post- infected（P 〈 0.01 ）. The MST was beyond 45 days for lethal infection group . Compared to normal controls, the expression of mRNA for IL - 10 increased markedly （3 d, P 〈0. 01 ; 7 d, P 〈0.01 ）. There were a large amount of fungi in kindeys of immunosupressed group and CFU in kidneys on 7 days were higher significantly than on 3 days post - infected （P 〈 0.01 ）. The MST was 14.2 days for immunosupressed group. Furthermore, the expression of mRNA for IL -10 and CFUs in immunosupressed group were significantly higher than those in sublethal doses group at the same time（3 d, P 〈0.01 ; 7 d, P 〈0.01 ; CFUs： 3 d, P 〈0.01, 7 d, P 〈0.01 ）.
出处
《岭南皮肤性病科杂志》
2005年第3期195-198,共4页
Southern China Journal of Dermato-Venereology
