摘要
以番茄环斑病毒阳性样品为研究材料,根据GeneBank(核酸序列数据库)中的序列设计特异性引物进行IC-RTP-CR(免疫反转录聚合酶链式反应,下同)扩增,获得产物克隆到pMD18-T载体中,经测序后与目标序列(ToRSVL19655)比较,核苷酸同源性为87.3%。通过实验建立了检测该病毒的IC-RT-PCR方法。该方法对其他样品核酸提取困难而抗体较容易制备的病毒检测同样具有借鉴意义。
Based on analyzing virus infected samples, a method for detecting tomato ringspot virus by IC-RT-PCR was developed. A special primer was designed according to the virus sequence in Genebank, IC-RT-PCR was done by using virus infected samples, IC-RT-PCR products （about 449 bp） were then cloned to the vector pMD18-T. Sequence analysis indicated that there is 87.3% identical between the cloned cDNA and the published virus sequence （ToRSV L19655）. The method provided a new approach for detecting other analogical virus.
出处
《中国植保导刊》
北大核心
2005年第9期8-10,共3页
China Plant Protection
