摘要
目的:为更好的控制康媛颗粒的质量。方法:采用薄层色谱法对当归、白芍和黄芪进行定性鉴别。采用高效液相色谱法对芍药菩进行含量测定,色谱柱为:Phenomenex Prodjgy ODS-3(4.6mm×150mm,5μm);流动相为:0.05mol/L 瞵酸二氢钾—乙腈(75:15);检测波长为230nm。结果:当归、白芍和黄芪的定性鉴别,阴性无干扰,斑点清晰,分离度良好。芍药苷在0.49—4.89μg 范围内具有良好的线性关系(r=0.9998),平均回收率为98.6%,RSD=0.97%。结论:方法简便,准确,重现性良好,可用于康媛颗粒的质量控制。
Objective： To control the quality of Kangyuan Granules. Methods： Radix angelicae sinensis, Radix paeoniae alba and Radix astragali of Kangyuan Granules were identified by ILC. Paeoniflorin of the Kangyuan Granules was determined by HPLC. Using a Phenomenex Prodigy ODS- 3（4.6omm× 150mm, 5μm）. The mobile phases consisted of 0.05mol/L KH2PO4 - AGN（75： 15）. The UV detector at 230nm. Results： The studies slaved that spot of naringoside on TLC can be well separated. The paeoniflorin was linear（r = 0.9998） within the range of 0.49 - 4.89μg, the average recovery was 98.6% with RSD=0.97%. Conclusions： The method established was simple, accurate and with good reproducibility. The method can be used for quality control of Kangyuan Granules.
出处
《黑龙江医药》
CAS
2005年第4期257-259,共3页
Heilongjiang Medicine journal
关键词
康媛颗粒
质量标准
当归
白芍
黄芪
中药制剂
Kangyuan Ganules
quality standard
TLC
HPLC
Radix angelicae sinensis
Radix paeoniae alba
Radix astragali
Paeoniflorin
