摘要
目的研究促甲状腺激素释放激素受体-2(TRH-R2)在大鼠睾丸出生后不同发育阶段的表达,探讨其在生殖发育调节中的作用。方法应用蛋白质免疫印迹杂交技术以及免疫组织化学ABC法检测8d、15d、20d、35d、60d和90d大鼠睾丸中TRH-R2的表达和定位,并结合图像分析技术对免疫组织化学结果进行统计学分析,观察其在发育过程中的变化。结果免疫印迹杂交发现TRH-R2蛋白表达于出生后15d以后各阶段的大鼠睾丸;而免疫组织化学在第8d以后的各个发育阶段均检测到TRH-R2的表达,TRH-R2定位于大鼠睾丸的间质细胞,TRH-R2免疫反应阳性物位于细胞膜和细胞质内,细胞核为阴性;图像分析结果显示随着大鼠睾丸的发育,TRH-R2表达量逐渐增多,35d达到最大值,此后维持稳定水平,表达量在35d同其他阶段之间具有统计学差异(P<0·01)。结论TRH-R2在大鼠睾丸发育过程中均有表达,定位于睾丸的间质细胞,其表达量随着增龄变化而变化,即同发育过程相关。
Objective To investigate the expression of thyrotrophin-releasing hormone receptor type-2（TRH-R2） in rat testis during postnatal development, and to discuss its function in the regulation of reproduction development. Methods Western blotting and the immunohistochemical ABC method were used to detect the expression and location of TRH-R2 in testes of 8,15, 20,35,60 and 90-day-old rats. And the immunohistochemical results were analyzed with the image analysis system and statistical method. Results Western blot showed that TRH-R2 was expressed in 15- to 90-day-old rat testis. As for the immunohistochemical ABC method,we found that TRH-R2 firsdy appeared in 8-day-old rat testes. In all the following stages we also detected the immunochemical positive cells in testes. TRH-R2 was distributed in the interstitial cells between seminiferous tubules and positive substance was expressed in the cell membrane and cytoplasm of Leydig cells. The results of image analyses quantitation revealed that the intensity of TRH-R2 increased progressively with the postnatal development of rats. The peak was in 35-day-old. Then at the following stages the expression of TRH-R2 maintained stable. Conclusion TRH-R2 is expressed in rat testis during its postnatal development and locates in the Leydig cells, and the TRH-R2 shows development-dependent expression in testicular tissue.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第4期407-411,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(39870109
30370750)
