摘要
目的探讨实验性蛛网膜下腔出血诱发脑血管痉挛时,内皮素受体-A(ETA)基因在大鼠脑基底动脉的表达变化及在诱发脑血管痉挛中的作用。方法56只Wistar大鼠随机分为正常组、蛛网膜下腔出血组(2、3、7、14d)、生理盐水组和假手术组,应用逆转录-聚合酶链反应(RT-PCR),以β-actin为内参照基因,分别检测各组实验动物脑基底动脉ETAmRNA的表达变化。结果蛛网膜下腔出血后2、3d组脑基底动脉ETAmRNA表达较正常组明显增高,7d组仍增高,14d组趋于正常。结论ETA在蛛网膜下腔出血诱发脑血管痉挛的病理生理过程中可能起重要作用。
Objective To detect the changes of Endothelin-A receptor mRNA in the cerebral basilar artery of rats with experimental subarachnoid hemorrhage-induced cerebral vessel spasm as well as its effect. Methods Fifty-six Wistar rats were randomly divided into normal, experimental, sham-operated and saline-control groups. The experimental group was subdivided into subarachnoid hemorrhage（SAH）2,3,7 and 14 d groups. Reverse transcription polymerse chain reaction was used to study the changes of Endothelin-A receptor mRNA level in the cerebral basilar artery of rats with experimental subarachnoid hemorrhageinduced cerebral vessel. Results ET-A receptor mRNA level of basilar artery in SAH2, 3 d and 7 d group was higher obviously than that of normal group, sham-operated and saline-control groups. That of 14 d group was approximate to normal group. Conclusion ET-A receptor may play an important role in pathogenesis of SAH-induced cerebral vessel spasm.
出处
《解剖学报》
CAS
CSCD
北大核心
2005年第4期400-402,共3页
Acta Anatomica Sinica
基金
卫生部科学基金(98-1-337)
江西省自然科学基金资助(0440062)
