摘要
目的:研究全反式视黄酸(ATRA)与IFN-α联合诱导HL-60细胞增殖分化的作用及其机制。方法:用5×10-7mol/L的ATRA以及2×106U/L的IFN-α单独和联合处理HL-60细胞后,采用POD-原位细胞凋亡检测试剂盒检测HL-60细胞的凋亡,通过计算细胞增殖的抑制率检测HL-60细胞增殖的抑制及诱导分化;用高效液相色谱(HPLC)检测细胞悬液中ATRA的含量;用原位杂交法检测细胞色素CYP26的表达。结果:ATRA和IFN-α均能诱导HL-60细胞分化、抑制其增殖并促进其凋亡,且二者具有明显的协同作用。IFN-α+ATRA联合处理组的细胞悬液中,ATRA的水平明显高于单纯ATRA处理组(P<0.05)。ATRA处理组CYP26表达的水平明显高于IFN-α单独处理组(P<0.05)。结论:ATRA+IFN-α诱导HL-60细胞的分化具有协同作用。其作用可能是由于IFN-α抑制了HL-60细胞内CYP26的表达,导致ATRA代谢速率减慢,使之保持了有效作用浓度所致。
AIM： To investigate the synergic effect of all-trans retinoic acid （ATRA） combined with IFN-α on the proliferation and differentiation of HL-60 cells. METHODS： HL-60 cell＇s differentiation and apoptosis were assessed by NBT reduction and TUNEL in siut apoptosis assay kit. CYP26 mRNA expression was detected by in situ hybridization assay kit. Metabolism of ATRA was measured by high performance liquid chromatography. RESULTS： Either IFN- α or ATRA induced HL-60 cell differentiation and apoptosis, which was enhanced when combining ATRA with IFN-α. The level of ATRA and the expression of CYP26 were higher in HL-60 cells treated with both ATRA and IFN-α than in the cells treated with ATRA alone. CONCLUSION： ATRA has remarkable synergic effect with IFN-α on HL-60 cells, proba- bly because IFN-α inhibits CYP26 mRNA expression and thus reduces the metabolism of ATRA.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第5期637-639,642,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
南阳市科技攻关项目基金资助(No.2004S006)
