摘要
基于猪附红细胞体广东株16S rRNA基因的序列特点,设计合成种特异性引物,建立了猪附红细胞体PCR检测方法。该方法能特异性扩增523 bp的猪附红细胞体16S rRNA基因片段,而对猪丹毒杆菌G 4T 10株、猪链球菌ST 171株、多杀性巴氏杆菌EO 630株、猪胸膜肺炎放线杆菌、猪肺炎支原体、鸡毒支原体和猫血巴尔通氏体CA株的基因组DNA没有扩增带出现。对猪附红细胞体基因组DNA的最小检测量为160 pg。通过对38份临床样品的检测,8份为猪附红细胞体感染阳性,其余为阴性。结果表明,建立的PCR检测方法具有极高的敏感性和特异性,可用于急性猪附红细胞体病和临床健康带菌猪的诊断。
Based on the sequence data of 16S rRNA gene of Eperythrozoon suis Guangdong strain,a set of E. suis-specific primers were designed. These primers selectively amplified a 522 bp DNA fragment of the 16S rRNA gene of E. suis Guangdong strain. No PCR products were amplified from purified DNA of Erysipelothrix rhusiopathiae,Streptococcus suis,Pasteurella multocida , Actinobacillus pleuropneuraoniae , Mycoplasraa hyopneuraoniae , Mycoplasraa gallisepticura and Haeraobartonella felis-CA strain. It was estimated that as few as 160 pg E. suis genomic DNA was detectable by PCR. Using this method,the 38 blood samples from different herds were successfully detected and 8 samples were positive with E. suis infection,the others were negative. The results showed that the PCR method developed was an effective tool for diagnosing E. suis infection in pigs with highly sensitive and specific. It will be useful for detecting the acute eperythrozoonosis and latent infected carrier animals.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2005年第5期480-483,共4页
Chinese Journal of Veterinary Science
基金
广东省自然科学基金资助项目(020388)
