摘要
实验用紫外线和丝裂霉素 C 作为机体 DNA 损伤的诱变剂,以人体外周淋巴细胞 DNA 非程序合成(UDS)水平、小鼠骨髓细胞诱发姐妹染色单体交换(SCE)频率为指标,分别观察了延缓衰老中药还精煎对人体及小鼠 DNA 损伤修复能力的影响。结果显示,老年人的 UDS 水平低于青年人,服用还精煎老人的 UDS 水平高于自身服药前水平,也高于未服药老寄:组,向青年人水平靠拢;饲喂还精煎小鼠的骨髓细胞诱发 SCE 值明显低于对照组。提示还精煎提高了机体对 DNA 损伤的修复能力,这可能就是药物补肾延衰作用的部分机理所在。
In order to observe the effect on organism DNA repair capacity after administration of Essence-Restoring Decoction(ERD),the level of peripheral lymphocyte unscheduled DNA synthesis (UDS)in 20 healthy young men,23 healthy old men and 13 old men given ERD were detected,while the frequency of sister chromosome exchange(SCE)of mice marrow cells in 80 ICR strain male mice were detected.Ultraviolet(UV)and mitomycin-C(MMC)were used as agents to induce injury of DNA.Experimental results showed that the level of UDS in the aged person was lower than that of the young person.Comparing the level of UDS of the aged before and after medication,it was shown that the level of UDS after medication was increased,approaching that of the young.Within a range of lower doses(<5mg/kg weight),the SCE value of mice marrow cells raised with the increase of MMC concentration,while this values was markedly lowered in the medicated mice as compared with that of the control.It is thus suggested that ERD could enhance the DNA repair capacity and this might be a part of the mechanism of'Invigorating Kidney and retarding aging'as indicated in TCM.
出处
《中西医结合杂志》
CSCD
北大核心
1989年第11期647-649,643,共3页
