摘要
从嗜热蓝藻优雅粘囊藻（Ｍｙｘｏｓａｒｃｉｎａ ｃｏｎｃｉｎｎａ Ｐｒｉｎｔｚ．）中分离到具有放氧活性的藻胆体－类囊体膜复合物。它的吸收峰位于６８０、６２８、４９０、４３８和４２０ ｎｍ ，在低离子强度（０．１～０．４ ｍ ｏｌ／Ｌ）磷酸缓冲液中的６６４ ｎｍ 荧光发射峰随离子强度的降低而升高，７１８ ｎｍ 荧光发射峰与此相反（７７ 。Ｋ，Ｅｘ＝ ５８０ ｎｍ ）。当把游离的藻胆体和已解离去藻胆体的类囊体膜在蔗糖磷酸缓冲液中重组时，随时间延长（０～６０ ｍ ｉｎ），７１８ ｎｍ 荧光发射峰逐渐升高，６８５ ｎｍ 荧光发射峰逐渐下降（７７ 。Ｋ，Ｅｘ ＝ ５８０ ｎｍ ）。
Ultrasonic treatment has been introduced to obtain photosynthetic vesicles with bound phycobilisome (PBS),the PBS thylakoid fraction,from Myxosarcina concinna Printz.The maximal oxygen evolving rate of PBS thylakoid membrane was around 60 μmol O 2/(mg Chl·h) and the optimum temperature was about 34 ℃.Absorption peaks of the complex were located at 680,628,490,438 and 420 nm.When the phycobiliprotein of the complex was excited by light at 580 nm,the fluorescence emission spectrum at room temperature exhibited a peak at 660 nm and a shoulder at 680 nm,while at liquid nitrogen temperature the peaks were resolved to 664,695 and 718 nm. The PBS bound with thylakoid membrane depolymerized and the energy transfer from PBS to thylakoid membrane decreased when the complex was put into a solution of low ion intensity.As the PBS was mixed with the PBS desociated thylakoid membrane,by contact with the thylakoid membrane the PBS could again transfer the energy trapped in itself to thylakoid membrane.
基金
国家自然科学基金
关键词
蓝藻
藻胆体
类囊体
能量传递
光能
Cyanobacteria
Phycobilisome
Thylakoid
Energy transfer
