摘要
目的:探讨Skp2和第10号染色体缺失的磷酸酶和张力蛋白同源物基因(Phosphatase and tensinhomologue deleted on chromosome 10,PTEN)在前列腺癌中的表达及临床意义。方法:用免疫组织化学EnVi-sionTM方法检测Skp2和PTEN蛋白在41例前列腺癌和20例BPH组织中的表达情况。结果:在前列腺癌中的Skp2蛋白染色阳性率显著高于BPH(P<0.01),Skp2蛋白表达与前列腺癌术前血清前列腺特异抗原(PSA)水平、局部浸润、肿瘤分期、病理分级呈密切正相关(P<0.05)。在前列腺癌中的PTEN蛋白染色阳性率显著低于BPH(P<0.01),PTEN蛋白表达与上述临床病理特征呈负相关(P<0.05)。前列腺癌中Skp2蛋白与PTEN蛋白表达呈负相关(P<0.01)。结论:Skp2蛋白在前列腺癌的发生发展中起重要作用,抑癌基因PTEN可能参与Skp2表达的调节。
Objective：To investigate expression of Skp2 and PTEN protein in human prostatic carcinoma and their clinical significance. Methods：Skp2 and PTEN protein expression were evaluated in the tissues of 41 human prostatic carcinomas as well as 20 benign prostatic hyperplasia （BPH） using immunohistochemistry （EnVision^TM method）. Results：The Skp2 labeling frequency in prostatic carcinoma was significantly higher than that in BPH（ P〈0.01）. The Skp2 protein expression in prostatic carcinoma was positively correlated with preoperative serum prostate-specific antigen level, extraprostatic extension, tumor stage, and histological grade（ P 〈0.05）. The PTEN labeling frequency in prostatic carcinoma was significantly lower than that in BPH （ P 〈0.01）. The Skp2 expression was inversely correlated with PTEN in prostate cancer （ P 〈0.01）. Conclusions： It indicates that Skp2 may play an important role in the development of human prostatic carcinoma and PTEN may regulate Skp2 expression.
出处
《临床泌尿外科杂志》
2005年第9期571-573,共3页
Journal of Clinical Urology
