摘要
目的建立测定盐酸精氨酸葡萄糖注射液中葡萄糖含量的方法。方法选择适宜的pH值,然后通过考察该pH值下溶液旋光度与盐酸精氨酸和葡萄糖浓度的线性关系并测定盐酸精氨酸的比旋度,建立旋光法测定葡萄糖含量的计算公式。结果测定pH值为4.2。在此pH值下,盐酸精氨酸和葡萄糖混合溶液的旋光度与盐酸精氨酸浓度和葡萄糖浓度的复相关系数为0.9999,线性范围分别为2.3～3.0g/100ml和1.8～3.2g/100ml;测得盐酸精氨酸的比旋度为11.54。结论盐酸精氨酸和葡萄糖的旋光度具有加和性,从测得的旋光度中扣除盐酸精氨酸的旋光度,即是葡萄糖的旋光度,葡萄糖的含量可按公式c=2.0852(α-0.1154c1)计算。
Objective To establish the method to determine the content of glucose in arginine hydrochloride and glucose injection by polariscopy. Methods The appropriate pH value was chosen first and the equation based on the linear relationship between the optical rotation and the concentrations of arginine hydrochloride and glucose and the messured value of the specific optical rotation of arginine hydrochloride was established. Results The pH value 4. 2 was appropriate. The measured value of specific optical rotation of arginine hydrochloride was 11.54. The multiple correlation coefficient between the optical rotation and the concentrations of arginine hydrochloride and glucose was 0.9999 with the linear range 2.3-3.0g/100ml of arginine hydrochloride and 1.8-3.2g/100ml of glucose. Conclusion The optical rotations of arginine hydrochloride and glucose possess additivity, the optical rotation of glucose can be obtained by subtracting the optical rotation of arginine hydrochloride from the total optical rotation. The assay of glucose in arginine hydrochloride and glucose injection can be computed from the formula as follow： c=2.0852 （ α -0.1154c1 ）.
出处
《食品与药品》
CAS
2005年第09A期31-33,共3页
Food and Drug
