摘要
根据鱼藤中异黄酮类化合物在紫外光２１０～２４５ｎｍ及２９０～３０５ｎｍ处具有吸收峰这一特性，提出了用紫外分光法测定鱼藤中异黄酮类总含量的方法．在３００ｎｍ波长处，浓度低于６×２．６１８×１０－３／ｍｏｌ·Ｌ－１时，测定了标准系列的吸光值，结果表明吸光值与浓度近乎直线相关，相关系数为０．９９７７；标准系列吸光值准确性高；加样回收率达９９％以上；测定样品时，重现性好．
According to the characteristic that the isoflavonoids of Derris elliptica Benth have a distinctive absorption spectrum within the ultroviolet wave length range (210￣ 345 nm, 290￣305 nm), a new method for determining the total isoflavonoids in Derris elliptica Benth by Ultroviolet Spectrometry has been put forward. With 300 nm wave length and concentration below 6x. 2618 × 10-3/mol· L-1,the absorption value of a standard series has been determined. The results indicate that the absorption value is related to the concentration with a straight-line correlation and the relative coefficient is 0. 9977;and that the absorption value of the standatd series is accurate; the recovery efficiency reachs 99% or more. When the sample is determined,the reappearing property of the spectrum is very good.
出处
《中南林学院学报》
CSCD
1996年第1期46-49,共4页
Journal of Central South Forestry University
关键词
鱼藤
异黄酮总量
紫外分光法
Derris elliptica Benth
the total of isoflavonoids
Ultraviolet spectrometry
