摘要
目的:研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体在胰腺癌中的表达及意义。方法:应用半定量RT-PCR,检测TRAILR mRNA在胰腺癌组织,正常胰腺组织及胰腺癌细胞系ASPC-1、Can-pan-2中的表达。结果:死亡受体DR4、DR5在所有胰腺癌组织、正常胰腺组织及胰腺癌细胞系中均有表达,诱骗受体DcR1、DcR2在所有正常胰腺组织及细胞系中均有表达。死亡受体DR4、DR5在胰腺癌组织中有较高的表达,而在正常胰腺组织中呈中低水平表达(P<0.01)。胰腺癌细胞系中死亡受体DR4、DR5呈高水平表达,而诱骗受体DcR1、DcR2仅呈中低水平表达。结论:TRAIL受体在胰腺癌普遍表达,并存在受体类型的表达差异;死亡受体在胰腺癌中高表达,可能在TRAIL诱导胰腺癌细胞凋亡的机制中发挥重要的作用。
Objeetive： To investigate the expression and significance of tumor necrosis Factor-related apoptosis-inducing Ilgand（TRAIL） recepors in pancreaitc carcinoma. Methods ： The expression of TRAILR mRNA was assayed by semiquantitive RT-POR In 30 pancretic carcinoma tlssues, 18 normal pancreatic tissues and 2 pancreatic cancer cell lines of ASPC-1,Oanpan-2. Reaults ：The expression of death receptor 4（DR4） and death receptor 5（DRS） was detected in all Dancreatic carcinoma tissues,normal pancreatic tissues and pancreatic cancer cell lines,decoy receptor （DcR1） and decoy reoeptor 2（DcR2） were expressed in normal pancreatic tissues and pancreatic ceil lines. The expression of DR4 and DR5 in the pancreatic carcinoma tissues was significantly higher than that in the normal pancreatic tissues（P〈0.0 1）. Couelusion ：TRAILR expression is prevalent in pancreatic tissues with different receptor types existing. High death receptor expresslon may play an important role in TRAIL inducing pancreatic carcinoma apoptosis.
出处
《中国现代普通外科进展》
CAS
2005年第5期281-283,共3页
Chinese Journal of Current Advances in General Surgery
