摘要
本实验在分离鉴定J亚群禽白血病JL_2株的基础上,利用PCR方法扩增出包括gp85基因在内的长度为960bp的DNA片段。将其连到PGEX_6p_1载体上,构建了重组表达质粒PGEX_6P-1/gp85,对质粒进行序列分析并在IPTG的诱导下进行表达。SDS_PAGE分析结果表明,融合蛋白(54 Ku)在BL21中得到有效表达。表达产物占菌体总蛋白的31.8%。Western Blot结果表明,表达产物可与ALV_J阳性血清发生特异性反应。这些结果为进一步研究gp85蛋白的功能及研制ALV_J ELISA诊断试剂盒奠定了基础。
A DNA fragment encoding gp85 of Avian Leukosis virus subgroup J JL-2 strain was amplified by PCR and cloned into PGEX-6P-1 expression plasmid. After sequencing, the recombined plasmid was transformed into E. coli BL21 （DE3） and induced by IFrG. SDS-PAGE analysis showed that the fusion protein was effjciently expressed. The result of Western Blotting also showed that the fusion protein expressed could react specifically with the ALV-1 positive serum.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2005年第5期356-359,共4页
Chinese Journal of Preventive Veterinary Medicine
